Cofilin-mediated actin filament network flexibility facilitates 2D to 3D actomyosin shape change.

The organization of actin filaments (F-actin) into crosslinked networks determines the transmission of mechanical stresses within the cytoskeleton and subsequent changes in cell and tissue shape. Principally mediated by proteins such as α-actinin, F-actin crosslinking increases both network connectivity and rigidity, thereby facilitating stress transmission at low crosslinking yet attenuating transmission at high crosslinker concentration. Here, we engineer a two-dimensional model of the actomyosin cytoskeleton, in which myosin-induced mechanical stresses are controlled by light. We alter the extent of F-actin crosslinking by the introduction of oligomerized cofilin. At pH 6.5, F-actin severing by cofilin is weak, but cofilin bundles and crosslinks filaments. Given its effect of lowering the F-actin bending stiffness, cofilin- crosslinked networks are significantly more flexible and softer in bending than networks crosslinked by α-actinin. Thus, upon local activation of myosin-induced contractile stress, the network bends out-of-plane in contrast to the in-plane compression as observed with networks crosslinked by α-actinin. Here, we demonstrate that local effects on filament mechanics by cofilin introduces novel large-scale network material properties that enable the sculpting of complex shapes in the cell cytoskeleton.

Intracranial Interdural and Extradural Cerebellar Convexity Dermoid Cyst: A Case Report of Rare Tumor at the Rarest Location.

Intracranial dermoid cysts are rare dysembryonic tumors of benign nature. These are uncommon in adults. If present, they are usually located in the midline or along the lines of embryonic fusion. The posterior fossa region is an infrequent site. Extradural or interdural locations are even more rare. In this case report, the authors report a laterally located large posterior fossa right cerebellar convexity interdural and extradural dermoid cyst over the sigmoid sinus. It was managed by totally extradural maximum possible safe decompression with microneurosurgical technique. The authors share their experience of addressing this rare pathology at the rarest location with unusual imaging findings.

A Rare Case Report of Simultaneous Occurrence of Chronic Subdural Hematoma and Acute Extradural Hematoma in a Patient after Multiple Ventriculoperitoneal Shunt Surgeries.

Ventriculoperitoneal (VP) shunt surgeries are common neurosurgical procedure for hydrocephalus. Subdural hematoma (SDH) and extradural hematoma (EDH) are rare but potentially life-threatening complications following VP shunt surgeries. We are describing probably the first case in which a 12-year-old boy presented to the emergency room in altered sensorium and gradual onset quadriparesis following multiple shunt revision procedures done in an outside hospital. Computed tomography head showed chronic right-sided frontotemporoparietal chronic SDH and parietal dominant EDH simultaneously with multiple shunt systems in ventricles. Patient was taken for surgery in emergency and emergent evacuation of hematoma was done. Patient improved in the postoperative period. In this case report we will make an attempt to describe a rare complication of VP shunt surgery and possible mechanisms responsible for it.

Quartet of catastrophe: Bilateral epidural hematoma in both supratentorial and infratentorial compartments - A case report and a novel surgical technique to approach.

Background: Epidural hematoma (EDH) is the most common form of traumatic brain lesion in the posterior fossa. This condition is rapidly fatal if not identified and treated accordingly, due to the proximity of the brain stem. Prompt diagnosis is made by early computed tomography (CT) of the head and emergent evacuation is of utmost importance. Case Description: A 28-year-old male presented to the emergency room with complaints of headache and vomiting following a road traffic accident. CT scan revealed EDH around the transverse sinus extending into supratentorial and infratentorial compartment bilaterally. The patient was planned for emergency surgery but relatives did not give consent initially they agreed after 24 h when the patient became unconscious. A midline incision was made and a small infratentorial craniectomy with two burr holes was made bilaterally above the transverse sinus. Excellent recovery was seen following a surgical procedure. Conclusion: Posterior fossa EDH is a rare but potentially fatal entity. Bilateral extension in supratentorial and infratentorial compartments makes it a "quartet of catastrophe." Prompt diagnosis and emergent evacuation lead to excellent recovery. Two burr holes in supratentorial compartments and a small infratentorial craniectomy can avoid sinus injury.

SPAK-dependent cotransporter activity mediates capillary adhesion and pressure during glioblastoma migration in confined spaces.

The invasive potential of glioblastoma cells is attributed to large changes in pressure and volume, driven by diverse elements, including the cytoskeleton and ion cotransporters.  However, how the cell actuates changes in pressure and volume in confinement, and how these changes contribute to invasive motion is unclear. Here, we inhibited SPAK activity, with known impacts on the cytoskeleton and cotransporter activity and explored its role on the migration of glioblastoma cells in confining microchannels to model invasive spread through brain tissue. First, we found that confinement altered cell shape, inducing a transition in morphology that resembled droplet interactions with a capillary vessel, from "wetting" (more adherent) at low confinement, to "nonwetting" (less adherent) at high confinement. This transition was marked by a change from negative to positive pressure by the cells to the confining walls, and an increase in migration speed. Second, we found that the SPAK pathway impacted the migration speed in different ways dependent upon the extent of wetting. For nonwetting cells, SPAK inhibition increased cell-surface tension and cotransporter activity. By contrast, for wetting cells, it also reduced myosin II and YAP phosphorylation. In both cases, membrane-to-cortex attachment is dramatically reduced. Thus, our results suggest that SPAK inhibition differentially coordinates cotransporter and cytoskeleton-induced forces, to impact glioblastoma migration depending on the extent of confinement.

Synthesis of Sulfonated Polyphenylene Block Copolymers via In Situ Generation of Ni(0).

Proton exchange membranes (PEMs) fabricated from sulfonated polyphenylenes (sPP) exhibit superior proton conductivity and electrochemical performance. However, the Ni(0) catalyst required for Colon's cross-coupling reaction for the synthesis of sPP block copolymers is expensive. Therefore, in this study, we generated Ni(0) in situ from an inexpensive Ni(II) salt in the presence of the reducing metal Zn and NaI. The sPP block copolymers were synthesized from neopentyl-protected 3,5- and 2,5-dichlorobenzenesulfonates and oligo(arylene ether ketone) using the catalyst NiBr2(PPh3)2. The block copolymers synthesized using our strategy and the Ni(0) catalyst exhibited comparable polydispersity index values and high molecular weights. Thin, transparent, and bendable PEMs fabricated using selected high-molecular-weight sPP block copolymers synthesized via our strategy exhibited similar proton conductivities to those of the block copolymers synthesized using the Ni(0) catalyst. We believe that our strategy will promote the synthesis of similar multifunctional block copolymers.

Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex.

The accumulation and transmission of mechanical stresses in the cell cortex and membrane determines the mechanics of cell shape and coordinates essential physical behaviors, from cell polarization to cell migration. However, the extent that the membrane and cytoskeleton each contribute to the transmission of mechanical stresses to coordinate diverse behaviors is unclear. Here, we reconstitute a minimal model of the actomyosin cortex within liposomes that adheres, spreads and ultimately ruptures on a surface. During spreading, accumulated adhesion-induced (passive) stresses within the membrane drive changes in the spatial assembly of actin. By contrast, during rupture, accumulated myosin-induced (active) stresses within the cortex determine the rate of pore opening. Thus, in the same system, devoid of biochemical regulation, the membrane and cortex can each play a passive or active role in the generation and transmission of mechanical stress, and their relative roles drive diverse biomimetic physical behaviors.

Recent trends and applications of evacuated tube solar collector in food processing and air heating: a review.

Solar energy demand is growing for future energy needs in different sectors to replace fossil fuels, which leads to a reduced carbon footprint and global warming. Evacuated tube solar collectors (ETSC) harness solar thermal energy for air heating, water heating, and drying in domestic and industrial sectors. The review paper comprises ETSC technology categorization, influencing factors like fin arrangement, integration of phase change material, tilt angle, solar radiation, and airflow rate on the performance of ETSC-based solar air heaters and dryers. The thermal performance parameters, like the collector efficiency, dryer efficiency, energy and exergy efficiency, thermal profile, zone temperature, relative humidity, heat loss during operations, etc., are reviewed. The developed ETSC-based air heating systems and solar dryers for drying agricultural products are performed effectively. However, research progress on improving the thermal performance integrated with nanofluids and phase change materials was discussed. CO2 mitigation analysis and global standards for ETSC-based air heaters and dryers are compiled. A large scope exists by use of solar air heaters (SAH) for food commodity drying with a suitable drying chamber and improving the designs of ETSC-based solar dryers. The work accomplished by various researchers has been analyzed in this study for prospective research gaps in the context of future design and development.

F-actin architecture determines constraints on myosin thick filament motion.

Active stresses are generated and transmitted throughout diverse F-actin architectures within the cell cytoskeleton, and drive essential behaviors of the cell, from cell division to migration. However, while the impact of F-actin architecture on the transmission of stress is well studied, the role of architecture on the ab initio generation of stresses remains less understood. Here, we assemble F-actin networks in vitro, whose architectures are varied from branched to bundled through F-actin nucleation via Arp2/3 and the formin mDia1. Within these architectures, we track the motions of embedded myosin thick filaments and connect them to the extent of F-actin network deformation. While mDia1-nucleated networks facilitate the accumulation of stress and drive contractility through enhanced actomyosin sliding, branched networks prevent stress accumulation through the inhibited processivity of thick filaments. The reduction in processivity is due to a decrease in translational and rotational motions constrained by the local density and geometry of F-actin.

Detailed Balance Broken by Catch Bond Kinetics Enables Mechanical-Adaptation in Active Materials.

Unlike nearly all engineered materials which contain bonds that weaken under load, biological materials contain "catch" bonds which are reinforced under load. Consequently, materials, such as the cell cytoskeleton, can adapt their mechanical properties in response to their state of internal, non-equilibrium (active) stress. However, how large-scale material properties vary with the distance from equilibrium is unknown, as are the relative roles of active stress and binding kinetics in establishing this distance. Through course-grained molecular dynamics simulations, the effect of breaking of detailed balance by catch bonds on the accumulation and dissipation of energy within a model of the actomyosin cytoskeleton is explored. It is found that the extent to which detailed balance is broken uniquely determines a large-scale fluid-solid transition with characteristic time-reversal symmetries. The transition depends critically on the strength of the catch bond, suggesting that active stress is necessary but insufficient to mount an adaptive mechanical response.

Synthesis of highly fluorescent and water soluble graphene quantum dots for detection of heavy metal ions in aqueous media.

Fluorescent graphene quantum dots (GQDs) are nanomaterials which possess unique properties that show great potential in different applications. In this work, GQDs were synthesized using graphene oxide (GO) as precursor via thermal treatment at high temperature. The obtained GQDs were highly fluorescent and were suitable for the determination of heavy metal ions. X-ray diffraction, FTIR spectroscopy, and UV visible spectroscopy confirm the formation of GQDs. TEM images show that formed GQDs have size ranging from 2 to 10 nm. Emission profile of aqueous GQDs was taken by exciting GQDs at different wavelength. The intensity of GQDs remains the same for 4-5 months. Furthermore, as prepared, GQDs were used for selective recognition of Fe3+, Pb+2, and Cr3+ from the bunch of different metal ions in aqueous media. Lower limit of detection obtained for Fe3+, Cr3+ and Pb2+ using GQDs were 50, 100 and 100 nM, respectively, which indicates that the GQDs can be utilized as a promising material for sensing of the heavy metal ions. Graphical abstract.

Boron nitride: a promising material for proton exchange membranes for energy applications.

Boron nitride (BN) is an exciting material and has drawn the attention of researchers for the last decade due to its surprising properties, including large surface area, thermomechanical stability, and high chemical resistance. Functionalization of BN is a new area of interest to build up novel properties and applications of BN. BN and functionalized BN are promising membrane materials and show enormous advantages ascribed to their simple synthesis, high surface area, mechanical and thermal stability, and distinctive mechanical properties. BN-based proton exchange membranes show improvement in their physicochemical, electrochemical, thermal, mechanical, and barrier properties. Only a few research studies have been carried out on BN-based highly stable proton exchange membranes (PEMs) for various electrochemical applications. In this review, we discuss the recent advances in the functionalization of BN by different methods. The synthesis of different proton exchange membranes has also been discussed in this article. In addition, the potential applications of hybrid proton exchange membranes have also been mentioned.

Filament Nucleation Tunes Mechanical Memory in Active Polymer Networks.

Incorporating growth into contemporary material functionality presents a grand challenge in materials design. The F-actin cytoskeleton is an active polymer network which serves as the mechanical scaffolding for eukaryotic cells, growing and remodeling in order to determine changes in cell shape. Nucleated from the membrane, filaments polymerize and grow into a dense network whose dynamics of assembly and disassembly, or 'turnover', coordinates both fluidity and rigidity. Here, we vary the extent of F-actin nucleation from a membrane surface in a biomimetic model of the cytoskeleton constructed from purified protein. We find that nucleation of F-actin mediates the accumulation and dissipation of polymerization-induced F-actin bending energy. At high and low nucleation, bending energies are low and easily relaxed yielding an isotropic material. However, at an intermediate critical nucleation, stresses are not relaxed by turnover and the internal energy accumulates 100-fold. In this case, high filament curvatures template further assembly of F-actin, driving the formation and stabilization of vortex-like topological defects. Thus, nucleation coordinates mechanical and chemical timescales to encode shape memory into active materials.

Entropy production rate is maximized in non-contractile actomyosin.

The actin cytoskeleton is an active semi-flexible polymer network whose non-equilibrium properties coordinate both stable and contractile behaviors to maintain or change cell shape. While myosin motors drive the actin cytoskeleton out-of-equilibrium, the role of myosin-driven active stresses in the accumulation and dissipation of mechanical energy is unclear. To investigate this, we synthesize an actomyosin material in vitro whose active stress content can tune the network from stable to contractile. Each increment in activity determines a characteristic spectrum of actin filament fluctuations which is used to calculate the total mechanical work and the production of entropy in the material. We find that the balance of work and entropy does not increase monotonically and the entropy production rate is maximized in the non-contractile, stable state of actomyosin. Our study provides evidence that the origins of entropy production and activity-dependent dissipation relate to disorder in the molecular interactions between actin and myosin.

Synthesis of Chloride-Free Potash Fertilized by Ionic Metathesis Using Four-Compartment Electrodialysis Salt Engineering.

A sustainable approach for the production of high-purity potash fertilizers devoid of chloride is highly needed. Conventional preparation processes for chloride-free potash fertilizers have certain limitations, such as complicated synthesis procedure, including high-temperature requirement, causing environmental pollution. In this work, a novel approach has been proposed for the production of high-purity potash fertilizer (KNO3, K2SO4, and KH2PO4) from KCl by metathesis electrodialysis (MED). Sulfonated poly(ether sulfone)-based cation-exchange membrane and quaternized brominated poly(2,6-dimethyl-1,4-phenylene oxide)-based anion-exchange membranes are used for the MED experiments. The membranes show adequate water uptake, ionic conductivity, and ion-exchange capacity with good mechanical and thermal stabilities. The yields of KNO3, K2SO4, and KH2PO4 are found to be 90, 86, and 90%, respectively. The power consumptions during MED experiment for KNO3, K2SO4, and KH2PO4 are calculated to 0.94, 0.89, and 1.04 kWh/kg, respectively. The purity of products is confirmed by inductively coupled plasma and X-ray diffraction analysis and by measuring ionic contents. The process provides an energy-intensive way for high-purity synthesis of KNO3, K2SO4, and KH2PO4.

Contractility in an extensile system.

Essentially all biology is active and dynamic. Biological entities autonomously sense, compute, and respond using energy-coupled ratchets that can produce force and do work. The cytoskeleton, along with its associated proteins and motors, is a canonical example of biological active matter, which is responsible for cargo transport, cell motility, division, and morphology. Prior work on cytoskeletal active matter systems showed either extensile or contractile dynamics. Here, we demonstrate a cytoskeletal system that can control the direction of the network dynamics to be either extensile, contractile, or static depending on the concentration of filaments or weak, transient crosslinkers through systematic variation of the crosslinker or microtubule concentrations. Based on these new observations and our previously published results, we created a simple one-dimensional model of the interaction of filaments within a bundle. Despite its simplicity, our model recapitulates the observed activities of our experimental system, implying that the dynamics of our finite networks of bundles are driven by the local filament-filament interactions within the bundle. Finally, we show that contractile phases can result in autonomously motile networks that resemble cells. Our results reveal a fundamentally important aspect of cellular self-organization: weak, transient interacting species can tune their interaction strength directly by tuning the local concentration to act like a rheostat. In this case, when the weak, transient proteins crosslink microtubules, they can tune the dynamics of the network to change from extensile to contractile to static. Our experiments and model allow us to gain a deeper understanding of cytoskeletal dynamics and provide an new understanding of the importance of weak, transient interactions to soft and biological systems.

Effect of aspect ratio on the development of order in vibrated granular rods.

We investigate ordering of granular rods in a container subject to vibrations in a gravitational field as a function of number density of the rods. We study rods with three different length to diameter aspect ratios A(r)= 5, 10, and 15. The measurements are performed in three dimensions using x-ray computer tomography to visualize the rods in the entire container. We first discuss a method to extract the position and orientation of the rods from the scans which enables us to obtain statistical measures of the degree of order in the packing. We find that the rods with A(r)=5 phase separate into domains with vertical and horizontal orientation as the number density of the rods is increased, whereas, for A(r)=10 and 15 the rods are predominately oriented vertically in layers. By calculating two-point spatial correlation functions, we further show that long range hexagonal order occurs within a layer when the rods are oriented along the vertical axis. Thus, our experiments find that long range order increases rapidly in granular rods with growing anisotropy.